Accuracy & precision of the PC100 platelet counter

ACCURACY OF THE PC100 FOR WHOLE BLOOD COUNTS

Accuracy is a measure of closeness of the agreement between the quantified analyte values obtained by the tested method/device and the true values of the gold reference method/device. Systematic error or inaccuracy is quantified by the average bias, which is usually expressed as the constant and proportional bias from a regression procedure. Following the CLSI EP9 method comparison guideline, the passing-bablok linear regression method was used to find the best mathematical fit between the quantified platelets obtained by PC100 and the gold reference device.

There is a close agreement between the quantified platelet values of PC100 and MUMC – Sysmex® XN-9000 Automated Hematology System. Results of regression analysis showed that the PC100 Platelet Counter can quantify blood platelets with an average bias (inaccuracy) of -3.362 (-2.312%) across the concentration range of 20-600 platelet/nanolitre. Accuracy is thus above 95%.

Accuracy of the PC100 platelet counter for whole blood counts
Accuracy of the PC100 platelet counter for whole blood counts

PRECISION OF the PC100 FOR WHOLE BLOOD COUNTS

Precision is a measure of closeness of agreement between quantified analyte values obtained by repeated measurements of the same sample/object under a specific set of conditions. Random error or imprecision is commonly expressed as the coefficient of variation (CV) calculated from the results of a set of duplicate measurements. Using duplicates for each sample, the CV% was calculated for the platelets of blood samples counted by PC100.

The PC100 Platelet Counter counts platelets with an average precision of 97.5% in the range of 20-600 platelet/nanolitre.

Precision of the PC100 platelet counter for whole blood counts
Precision of the PC100 platelet counter for whole blood counts

ACCURACY OF THE PC100 FOR PRP COUNTS

There is close agreement between the quantified platelet values of the PC100 Platelet Counter and Sysmex® XP-300 Automated Hematology System. Results of regression analysis showed that the PC100 Platelet Counter can quantify PRP platelets with an average bias (inaccuracy) of (2.0%) across the concentration range of 250-3600 platelet/nanolitre. Accuracy is thus above 95%.

Accuracy of the PC100 platelet counter for PRP counts